Feline N-terminal Pro-brain Natriuretic Peptide Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)(fNT-proBNP)

[Ingoa Hua]

Ingoa: feline NT-proBNP whakamātautau kotahi taahiraa

 

[Whakaahuatanga Taake]

10 nga whakamatautau / pouaka


Taipitopito Hua

Tohu Hua

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Whānui

I te wa o te hunanga, ka wehea te pūmua ki te BNP (77th ki te 108th waikawa amino) me te wahanga n-terminal NT-proBNP(1st ki te 76th amino acids).Ina hunahia te BNP, e 32 waikawa amino te roa, ki roto i te toto, ka herea e ia ki ona kaiwhakawhiwhi (NPRA me te NPRB) me te whakahaere i te pehanga toto na roto i nga momo tikanga.Ko te NT-proBNP, he 76 waikawa amino te roa, kaore he mahi koiora, engari na te mea he roa ake te haurua o te ora i te BNP, he pai ake hei tohu tohu mo nga momo mate ngakau.I roto i nga whakamatautau haumanu o nga kararehe, ko te toto o te NT-proBNP i roto i nga kuri he teitei ake i te 900 pmol/L, me nga ngeru kei runga ake i te 270 pmol/L, a he nui te mate o nga mate e pa ana ki te ngakau.Engari, me tohu na te mea ka peia te NT-proBNP e te whatukuhu, i te wa e pa ana te kararehe ki nga mate e pa ana ki te whatukuhu, ka piki ake ano te kukū o te NT-proBNP i roto i te tinana, ka puta mai he hua teka i runga i te whakamatautau.

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Tikanga Rapu

Ko tenei hua ka tango i te immunochromatography fluorescence kia kitea te kiko o te fNT-proBNP i roto i te serum/plasma ine ine.Tikanga taketake: He rarangi T me C kei runga i te kiriuhi muka waikawa nitric, ka pania te raina T ki te antibody a e mohio ana ki te fNT-proBNP.Ka rehuhia te papa huinga ki tetahi atu mea nano karekoata kua tapaina he antibody b ka taea te tautuhi i te FDT-probNP.I roto i te tauira, ka hono tuatahi te FDT-probNP me te nanomaterial kua tapaina te antibody b ki te hanga i te matatini, ka ki te chromatography o runga, ka hono te matatini ki te T-line antibody A ki te hanga i te hanganga hanawiti.A, no te te excitation o te irradiation marama, te nanomaterial emits tohu fluorescence.Ko te kaha o te tohu he pai te hono ki te kukū o te fNT-proBNP i roto i te tauira.


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