Kete Ine Tairoxine Tapeke a Canine (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)(cTT4)

[Ingoa Hua]

Tiroxine Tapeke Canine (cTT4) Kete Whakamātautau (cTT4 Whakamātautau kotahi taahiraa)

 

[Whakaahuatanga taake]

10 nga whakamatautau / pouaka


Taipitopito Hua

Tohu Hua

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Te Kaupapa Whakamatau

Ko te T4 te hua matua o te hunanga taikaro, a he waahanga ano hoki e kore e taea te tuhi i te pono o te punaha ture hypothalamic-anterior pituitary-thyroid. Ka whakanui ake i te reiti metabolic basal me te whai waahi nui ki te whanaketanga o nga pūtau tinana katoa. Kei te rongoa a T4 i roto i nga pukoro thyroid i te taha o te thyroglobulin, ka hunahia, ka tukuna i raro i te ture a te TSH. Neke atu i te 99% o T4 i roto i te serum kei te ahua o te here ki etahi atu pūmua. Ko te whakamatautau mo te T4 katoa i roto i te tauira toto ka mohio mena kei te kino te mahi o to tairoi.

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Tikanga Rapu

Ka whakamahia e tenei hua te immunochromatography fluorescence ki te kitea ine ine te ihirangi o te cTT4 i roto i te serum kuri / plasma. Tikanga taketake: Ko nga rarangi T me C kua tohua ki te kiriuhi nitrocellulose, kua pania te raina T ki te cTT4 antigen a, ka rehuhia te papa here ki te nanomaterial fluorescent tapanga antibody b e mohio ana ki te cTT4. Ko te cTT4 i roto i te tauira ka tapaina tuatahi ki te nanomaterial. Ka herea te antibody b ki te hanga i te matatini, katahi ka chromatograph ki runga. Ka whakataetae te matatini ki te antigen a T-line a kaore e taea te hopu; Engari, karekau he cTT4 i roto i te tauira, ka herea te antibody b ki te antigen a. I te wa e whiti ana te rama whakaihiihi, ka tukuna e te rauemi nano he tohu rewharewha, a, ko te kaha o te tohu he rereke ki te kukū o te cTT4 i roto i te tauira.


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