Te tangohanga waikawa karihi me te miihini rapu a New Tech

E rima te kaha:
● He taputapu i whirihorahia me te tangohanga waikawa karihi me te hikoi parakore
● Taputapu whirihora ki te kōwae tangohanga ultrasonic
● Taputapu whirihora ki te tino aunoa
● Taputapu i whirihorahia me te whakanui i te pāmahana rerekee
● He taputapu i whirihorahia me te kete reagent kua oti te kati

1.Me tango, me pure nga reagents kimi waikawa karihi?
Ko te maataapono o te kimi waikawa karihi e whai ake nei: i raro i te mahi a te tuatahi, ka whakamahia te DNA polymerase ki te whakanui i te tauhohenga mekameka ki runga i te tauira DNA/RNA (e hiahia ana ki te whakawhiti whakamuri o te NA), katahi ka kitea te nui o te tohu rewharewha i tukuna hei whakatau. mehemea kei roto i te tauira te waikawa nucleic (DNA/RNA) o te tukumate kia kitea.

1) Ko nga tauira kaore ano kia tangohia, kia purea ranei he maha nga waahanga e pa ana ki te hua whakamutunga: te karihi (ka taea te whakakore i te waikawa nucleic i whngaia ka puta he kino teka ), protease (ka taea te whakaiti i te DNA polymerase me te hanga kino kino), te whakarewa taimaha. te tote (e arai atu ai ki te kore whakakorikori o te synthase ka puta he pai teka ), he waikawa rawa, he kawakore rawa ranei te PH (e kore ai te tauhohenga e taka), RNA Inooti (e arai ana ki te koretake o te tuhi whakamuri kino).

2) He uaua etahi tauira ki te whakakaha tika: Gram-positive me etahi pirinoa, na te matotoru o o ratou pakitara pūtau me etahi atu hanganga, ki te kore e uru ki te tangohanga waikawa karihi me te mahi purenga, ka kore pea te kete tangohanga-kore mo tera. tauira.

No reira, e taunaki ana kia kowhiria te kete whakamatautau, taputapu ranei i whiria ki te taahiraa tangohanga waikawa karihi.

2. Tangohanga matū, tangohanga wehenga ultrasonic tinana ranei?
Ko te tikanga, ka taea te tono tangohanga matū ki te nuinga o te maimoatanga o mua me te Puri.Heoi, i roto i nga huakita Gram-positive-taiepa matotoru me etahi pirinoa, he pera ano te take kaore e taea e te tangohanga matū te whiwhi tauira waikawa karihi whai hua, ka puta he kitenga kino.I tua atu, he maha nga wa e whakamahia ana e te tangohanga matū nga kaihoko kaha, ki te kore e tino rite te elution, he ngawari te whakauru i te alkali kaha ki roto i te punaha tauhohenga, ka puta he hua kore.

Ko te wehenga o te Ultrasonic e whakamahi ana i te kuru tinana, he mea pai te whakamahi a GeneXpert, he hinonga rangatira i te mara o POCT mo te whakamahi tangata, a he tino painga ki te tangohanga waikawa karihi o etahi tauira uaua (pēnei i te Mycobacterium tuberculosis).

No reira, e taunaki ana kia whiriwhiria he kete whakamatautau, he taputapu ranei i whirihorahia ki te waahanga tangohanga waikawa karihi.a he mea tino pai mena he waahanga tangohanga ultrasonic.

3. Manual , Semi-aunoa me te tino aunoa?
He raruraru tenei mo te utu mahi me te kaha mahi.I tenei wa, ko nga hohipera mokai kaore i te nui nga kaimahi, me te tangohanga me te kimi waikawa karihi he mahi e hiahia ana etahi pukenga me etahi wheako.Karekau he whakaaro ko te tangohanga waikawa karihi aunoa me te miihini rapunga te mea tino pai.

4. Te whakanui ake i te pāmahana, te whakanui i te pāmahana rereke ranei?
Ko te tauhohenga whakakaha he hononga kimi waikawa karihi, he uaua te hangarau ngaio kei roto i tenei hononga.Ko te tikanga, ka whakamahia nga enzymes hei whakanui i te waikawa karihi.I roto i te tukanga whakakaha, ka kitea te tohu rewharewha whakakaha, te tohu rewharewha whakauru ranei.Ko te tikanga, ka puta wawe te tohu rewharewha, ka nui ake te ihirangi ira o te tauira.

Ko te whakakaha ake o te pamahana he whakakaha waikawa karihi i te pāmahana pumau, engari ko te whakakaha o te pāmahana taurangi he whakahiatotanga huri noa i runga i te denaturation-annealing-toronga.Ko te wa whakakaha ake o te pamahana kua mahia, ko te wa whakamaarama rerekee e pa ana ki te tere o te piki me te heke o te taputapu (i tenei wa, he maha nga kaihanga kua kaha ki te mahi i nga huringa 40 o te whakakaha i roto i te 30 meneti).

Mena he pai nga tikanga taiwhanga me te kaha o te roherohenga, he tika te kii karekau e tino nui te rerekeetanga tika i waenga i nga mea e rua.Heoi, ka whakahiatohia e te kaha o te pāmahana rerekee nga hua waikawa nucleic i roto i te wa poto ake.Mo nga taiwhanga karekau he tino roherohe me nga kaimahi whakangungu ngaio, ka nui ake te mate o te rerenga aerosol waikawa nucleic, ka puta te pai o te teka ina pa mai te rerenga, he tino uaua ki te whakakore.

I tua atu, ka kaha ake te whakakaha ake o te pamahana ki te whakakaha kore-motuhake ina he uaua te tauira (he iti ake te pāmahana tauhohenga, me te teitei ake o te pāmahana toronga, ka pai ake te herenga tuatahi).

Mo nga hangarau o naianei, he pono ake te whakakaha o te pāmahana rerekee.

5. Me pehea te karo i te morearea o te rerenga o nga hua whakakaha waikawa nucleic?
I tenei wa, he maha nga kaihanga e whiriwhiri ana i te ngongo PCR momo repe rite te ngongo tauhohenga waikawa karihi, ka hiritia e te waku, me te denaturation pāmahana i roto i te denaturation pāmahana rerekē i roto i te taurangi pāmahana PCR whakanuinga tae 90 tohu.
Centigrade .He wero nui ki te hiri o te ngongo PCR, he uaua ki te hiri o te ngongo PCR, he ngawari noa te pakaru o te ngongo PCR momo repe.

He pai ake ki te tango i te tauhohenga me te kete hiri / ngongo kia kore e pakaru te hua tauhohenga.He tino pai mena ka mahia he kete kua oti te hiri mo te tangohanga me te kimi waikawa karihi.

No reira, ko te tangohanga waikawa karihi aunoa hou a New Tech me te miihini rapu i nga whiringa pai e rima o runga ake nei.